Presentations & Publications

Airborne particulate concentration during non-thermal nano-pulse stimulation wart clearance is negligible compared to thermal modalities

Authors: Edward V. Ross, James Newman, Jayachandran Ravichandran, Richard Nuccitelli
Published in Lasers in Surgery and Medicine, online August 2021

View article >

Safety and Efficacy of Nano-Pulse Stimulation Treatment of Non-Genital, Cutaneous Warts (Verrucae)

Authors: Richard Nuccitelli PhD, Brenda M. LaTowsky MD, Edward Lain MD, Girish Munavalli MD, Lesley Loss MD, E. Victor Ross MD, Lauren Jauregui BS, William A. Knape BS
Published in Lasers in Surgery and Medicine, online May 2021

View article >

A dose-response study of nanosecond electric energy pulses on facial skin

James Newman, MD, Lauren Jauregui, William A. Knape, BS, Edward Ebbers, BBA, MBA, Darrin Uecker, MS, Darius Mehregan, MD, Richard Nuccitelli, PhD
Published in Journal of Cosmetic and Laser Therapy, DOI: 10.1080/14764172.2020.1827151

View article >

Safety and Efficacy of Nanosecond Pulsed Electric Field Treatment of Sebaceous Gland Hyperplasia

Girish S. Munavalli, MD, Brian D. Zelickson, MD, Mona M. Selim, MD, Suzanne L. Kilmer, MD, Thomas E. Rohrer, MD, James Newman, MD, Lauren Jauregui, BS, William A. Knape, BS, Edward Ebbers, BS, Darrin Uecker, MS, and Richard Nuccitelli, PhD
Published in Dermatologic Surgery 2020; 46: 803–809

View article >

Safety and Efficacy of Nanosecond Pulsed Electric Field Treatment of Seborrheic Keratoses

George J. Hruza, MD, MBA, Brian D. Zelickson, MD, Mona M. Selim, MD, Thomas E. Rohrer, MD, James Newman, MD, Hyoung Park, BS, MBA, Lauren Jauregui, BS, Richard Nuccitelli, PhD, William A. Knape, BS, Edward Ebbers, BBA, MBA, and Darrin Uecker, MS
Published in Dermatology Surgery, online December 2019

View article >

Nano-Pulse Stimulation Therapy for the Treatment of Skin Lesions

Richard Nuccitelli, PhD
Published in Bioelectricity, online December 2019

View article >

A Dose‐Response Study of a Novel Method of Selective Tissue Modification of Cellular Structures in the Skin With Nanosecond Pulsed Electric Fields

David Kaufman, MD, Michelle Martinez, RN, SSN, Lauren Jauregui, BS, Edward Ebbers, BAA, MBA, Richard Nuccitelli, PhD, William A. Knape, BS, Darrin Uecker, MS, and Darius Mehregan, MD
Published as Editor’s Choice in Lasers in Surgery and Medicine 2019; 52: 315-322.

View article >

First Clinical Use of Non-Thermal Nano-Pulse Stimulation Procedure to Eliminate Sebaceous Hyperplasia Lesions

S. Kilmer, MD, E. Ebbers, L. Jauregui
2019 American Society for Laser Medicine and Science (ASLMS) Annual Meeting (March 2019)

High Amplitude, Nanosecond Electrical Pulsing for Warts

Vic Ross, MD
2019 American Society for Laser Medicine and Science (ASLMS) Annual Meeting (March 2019)

Safety and Efficacy of Nano-Pulse Stimulation in the Treatment of Patients with Sebaceous Hyperplasia

G. Munavalli, MD, A. Macri, L. Jauregui, D. Uecker, W. Knape, E. Ebbers
2019 American Academy of Dermatology (AAD) Annual Meeting (March 2019)

Histology of Cellular Sebaceous Glands After Nano-Pulse Stimulation Procedure Demonstrates Mechanism for High Sebaceous Hyperplasia Clearance Rate in Clinical Use

S. Kilmer, MD, J. Newman, MD, E. Ebbers, L. Jauregui, H. Park, D. Mehregan, MD
Maui Derm for Dermatologists Annual Meeting (January 2019)

A Non-Thermal Method for Clearing Seborrheic Keratoses Using a Novel Nano-Pulse Electrical Energy Source

G. Hruza, MD, H. Park, P. Buckman, MSN
2018 American Society for Dermatologic Surgery (ASDS) Annual Meeting (October 2018)

A Dose-Response Study of a Novel Non-Thermal Method of Selectively Modifying Cellular Structures in Skin with Low Energy Nanosecond Electrical Stimulation

NPS™ technology was found to provide a safe, non-thermal mechanism for targeting cellular structures with a mild inflammatory response
Positive Caspase-3 staining at 2 and 4 hours post-NPS treatment confirms Programmed Cell Death (PCD)

Presented at American Society for Laser Medicine and Surgery Annual Meeting (2018) and received the Best of Basic Science and Translational Research Award.

View study poster >

Poster: A Dose-Response Study of a Novel Non-Thermal Method of Selectively Modifying Cellular Structures in Skin with Low Energy Nanosecond Electrical Stimulation

First Clinical Use of Non-Thermal Nano-Pulse Stimulation™ Treatment to Eliminate Seborrheic Keratosis Lesions

82% of treated SK lesions rated by investigators as cleared or mostly cleared with a single NPS treatment
Evidence to guide appropriate energy parameters and protocol design decisions for planned future clinical studies of other benign and non-benign lesions

Presented at American Society for Laser Medicine and Surgery Annual Meeting (2018).

View study poster >

Poster: First Clinical Use of Non-Thermal Nano-Pulse Stimulation to Eliminate Seborrheic Keratosis Lesions

Nano-Pulse Stimulation™ technology induces immunogenic cell death in human papillomavirus-transformed tumors and initiates an adaptive immune response

PloS One, 2018;13(1):e0191311

View PDF >

Profiling the immunogenic cell death (ICD) mechanisms induced by Nano-Pulse Stimulation (NPS) treatment in mouse B16-F10 melanoma tumors using NanoString technology

Poster Presentation: Society of Immunotherapy of Cancer Annual Scientific Meeting, November 2017

View article >

Nano-Pulse Stimulation™ technology is a physical modality that can trigger immunogenic tumor cell death

• Richard Nuccitelli Email author
• Amanda McDaniel
• Snjezana Anand
• John Cha
• Zachary Mallon
• Jon Casey Berridge
• Darrin Uecker

Nanosecond pulsed electric field treatment of tumor cell lines triggers immunogenic cell death (ICD)

Poster Presentation: Society of Immunotherapy of Cancer Annual Scientific Meeting, November 2016

Nano-Pulse Stimulation treatment induced three markers of ICD in three tumor cell lines, providing a possible explanation of why in vivo NPS treatment has shown a vaccine-like effect that inhibits secondary tumor growth after subsequent challenges with tumor cells.

View PDF >

Nanosecond pulsed electric field treatment of murine melanomas initiates an immune response and inhibits metastasis

Poster Presentation: Society of Immunotherapy of Cancer Annual Scientific Meeting, November 2016

Mice given a single melanoma tumor which were later either treated surgically or with Nano-Pulse Stimulation treatment. When subsequently challenged with intravenously injected melanoma cells, the NPS-treated mice exhibited fewer metastases than did the mice that had surgical removal, suggesting that the immune system had removed some of the melanoma cells from circulation in the NPS treated mice. This observation extends NPS’s vaccination effect beyond secondary tumor challenge to circulating cancer cells.

View PDF >

Adaptive immune response to Nano-Pulse Stimulation

Poster Presentation: Society of Immunotherapy of Cancer Annual Scientific Meeting, November 2016

Nano-Pulse Stimulation treated murine fibrosarcoma tumors significantly increased the infiltration of immune system cells into the treated primary tumor and three weeks later challenge tumors were completely rejected due to the production and increase in functionality of T cells.

View PDF >

Nanosecond pulsed electric field treatment of murine melanomas initiates an immune response and inhibits metastasis.

Poster Presentation: Society of Immunotherapy of Cancer Annual Scientific Meeting, November 2016

Mice given a single melanoma tumor which were later either treated surgically or with Nano-Pulse Stimulation treatment. When subsequently challenged with intravenously injected melanoma cells, the NPS-treated mice exhibited fewer metastases than did the mice that had surgical removal, suggesting that the immune system had removed some of the melanoma cells from circulation in the NPS treated mice. This observation extends NPS vaccination effect beyond secondary tumor challenge to circulating cancer cells.

View PDF >